polyclonal antibodies against mdr1 (multi drug resistance 1 Search Results


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NSJ Bioreagents mdr1 antibody multidrug resistance 1
Mdr1 Antibody Multidrug Resistance 1, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
This monoclonal antibody reacts with p-glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells with secretory and excretory functions and is assumed to function as an ATP-dependent drug efflux pump with
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Cell Signaling Technology Inc akt
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
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Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc cleaved caspase 3
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc β actin
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc cleaved caspase 9
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LI-COR odyssey imaging system
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs protoscript first strand cdna synthesis kit
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
Protoscript First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LI-COR odyssey
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hct-8  (ATCC)
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ATCC hct-8
Berberine <t>inhibited</t> <t>PI3K/AKT/mTOR</t> signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).
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Image Search Results


Berberine inhibited PI3K/AKT/mTOR signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).

Journal: Frontiers in Pharmacology

Article Title: Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer

doi: 10.3389/fphar.2020.616251

Figure Lengend Snippet: Berberine inhibited PI3K/AKT/mTOR signaling in the DDP-resistant gastric cancer cells with cisplatin treatment. (A,B) Western blot determined phosphor-PI3K, PI3K, phospho-AKT, AKT, phosphor-mTOR or mTOR protein levels in BGC-823/DDP and SGC-7901/DDP cells after treatment with DDP (30 µM), Berberine (30 µM) or DDP (30 µM) + Berberine (30 µM). * p < 0.05 compared to Blank group; # p < 0.05 compared to DDP (30 µM) group; $ p < 0.05 compared to berberine (30 µM) group ( n = 3).

Article Snippet: The concentrations and the sources of the primary antibodies were shown below: β-actin (1:2,000; Cell Signaling Technology, Danvers, United States), cleaved caspase-3 (1:1,000; Cell Signaling Technology), cleaved caspase-9 (1:1,000, Cell Signaling Technology), Bax (1:1,000; Cell Signaling Technology), multidrug resistance-associated protein 1 (MRP1; 1:1,000; Cell Signaling Technology), multi-drug resistance-1 (MDR1; 1:1,500; Cell Signaling Technology), phosphorylated (phospho)-PI3K (1:1,000; Cell Signaling Technology), PI3K (1:1,000; Cell Signaling Technology), phospho-AKT (1:1,000; Cell Signaling Technology), AKT (1:1,000; Cell Signaling Technology), phospho-mTOR (1:1,000; Cell Signaling Technology) and mTOR (1:1,000; Cell Signaling Technology).

Techniques: Western Blot